新的β-二氢沉香呋喃倍半萜多元酯和生物碱的HR-FABMS研究

利用高分辨快原子轰击质谱(HR-FABMS),以(甘油 氯化钠)为基质测定了9个新的β-二氢沉香呋喃倍半萜多酯和生物碱的HR-FABMS。结果表明,所有该类化合物均可以精确地获得[M Na] ,[M H] ,[M H-H2O] 等特征离子的质量和元素组成,从而方便地获得原有该类化合物的分子量和分子式。     

用Auto CAD2000进行机械二维图形的设计

叙述了用AutoCAD2 0 0 0进行机械二维图形设计的各种技巧 ,并详细介绍了绘图时的环境设置、图形绘制、标注及绘图输出等。     

犬病毒性肝炎的诊断与防制

详细闸达了犬病毒性肝炎的发病规律,临床症状,剖检变化以及实验室检查结果,提出了该病的具体防治措施,并介绍了犬病毒性肝炎疫苗,抗血清,康复犬的全血清,丙种球蛋白时预防本病有较好的效果。     

论生态旅游、生态旅游系统及其基本特征

文章通过研究生态旅游概念,探讨了生态旅游系统组成要素及其本质内涵与特征。     

Utilization of Aromatic Rice in Improving Grain Quality of Hybrid Rice

ThesuccessfuldevelopmentandcommercialuseofhybridricehasgreatlyraisedriceyieldlevelinChina .However ,ithasbeenagreatchallengeforbreederstoim provethegrainqualityofhybridricewithoutsacrificeofitsyield ,i.e .,todevelophybridricecombinationswithgoodgrainqualityaswellashighyield .AromaticricelikeBasmatiinIndiaandPakistanandJasmineinThailand ,isfamousforitshighqualityandu niquepleasantflavorofaroma ,soitishighlyfavoredbyriceconsumersaroundtheworld .Itmightbeaneffectiveapproachtoimprovethegrainqual…     

优良乡土树种观光木选育与栽培技术研究

观光木是木兰科单种属植物，是国家保护的珍稀濒危植物，是我国南方常绿阔叶林中的优势建群树种。以其树干通直、材质优良、速生、树形优美等特性而备受关注。我们在观光木科学引种栽培的基础上，对其生物生态学特性、繁育技术、营林配套技术进行了一系列基础性的研究工作，为全面选育和开发该树种奠定了良好的技术支撑。     

野生朱红硫磺菌驯化栽培研究

营养试验、菌丝体培养和出菇温度试验、培养基酸碱度试验、出菇条件试验结果表明：朱红硫磺菌菌丝体适宜生长的温度范围为15-32℃，最适温度范围为22～28℃；子实体发生的最适温度为16～25℃，气温低于16℃，子实体色泽为淡橙色，16℃以上，子实体呈鲜橙红色；培养料适宜营养C／N为20-26／1；朱红硫磺菌菌丝体适宜酸碱度范围为pH3．0～8．0，最适酸碱度为pH4．0—6．5；朱红硫磺菌的产量与光照条件无关，但光照强度影响子实体的发生时间和色泽；朱红硫磺菌栽培原料广泛，凡栽培木腐菌的培养料均可用于栽培朱红硫磺菌。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

Effect of CGRP on level of lung endogenous NO in rabbits with acute lung injury

AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI.     

The transdifferentiation of Sca-1+ cells from murine fetal liver

AIM:To explore transdifferentiation potential of Sca-1⁺ cells from murine fetal liver. METHODS:2×10³ of Sca-1⁺ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from ⁶⁰ Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA⁺/CD₄₅^-F_4/80^- and NueN⁺/CD₄₅^-F_4/80^-, respectively. CONCLUSION:The evidence is provided for Sca-1⁺ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells.